FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5827546
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA1400.057.08030743
CGCGTAT1700.049.05321544
GATCGGA10000.048.988531
ATCGGAA11100.045.6925662
ACACGTC11300.044.56762313
AATCGCG3000.044.00332341
ACTCCGG11450.043.68380732
CCGGAGA11500.043.49535
TCGGAAG11700.043.346013
CACTCCG11550.043.0044931
CGGAGAA11700.042.75222836
CGTCTGA11950.042.725116
CTCCGGA12200.040.99691833
TATGCCG4100.040.68630648
TCCGGAG13750.036.6286834
CGGAAGA14250.035.589354
ACGTCTG14350.035.5794415
ATCGCGT2550.035.42397342
GAATCGG3700.034.7385740
CACGTCT15350.033.714114
Produced by FastQC (version 0.11.5)