FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6946871
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA77820.11202165694454382TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG11300.053.19935648
GATCGGA11700.052.247851
CGCTCAT11900.051.36417834
CTCGTAT11750.050.55771344
TCTCGTA11950.049.7097743
CGTATGC12350.049.51222246
TATCTCG12200.048.6865741
TGCCGTC12500.048.37927250
ACCGCTC12900.047.38280532
GTCACCG12900.047.37904729
ATCGGAA13300.046.219882
TCGTATG13450.045.71758745
ATGCCGT13200.045.54548649
GCCGTCT13550.044.89135451
ATCTCGT13200.044.73729742
CACCGCT13900.044.22254631
ACACGTC14050.043.99347713
CCGCTCA14050.043.5038733
TCGGAAG14350.043.07813
CGTCTTC14900.041.06321753
Produced by FastQC (version 0.11.5)