FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8094085
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6500.042.76219648
TGCCGTC7950.034.96993650
GCCGTCT8050.034.5387351
CGTATGC8300.033.9018346
TCTCGTA8100.033.01728443
CTCGTAT8550.031.2820544
ATGCCGT9050.030.71640849
GTCACGA9900.030.15765629
GATCGGA8650.030.109461
ACACGTC10050.029.01666813
CGTCTGA10050.029.01666816
ACGGCGA2050.028.793992
ACGAGAT10100.028.53176332
TCGTATG9900.028.4216945
CGTCTTC10600.026.89162353
CCGTCTT10850.026.26972452
AGTCACG11000.026.1950828
ATCTCGT10100.025.4470842
ATCGGAA11300.025.1966132
TCGGAAG11650.024.4393333
Produced by FastQC (version 0.11.5)