FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8598269
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCGT3900.017.80951524
TCGGCCG4900.014.17475523
TGGTCGG5800.013.17297620
GGTCGGC5750.012.6838421
GCCGTCA6301.8189894E-1211.57645326
CGGACAC4050.001123646311.23241370
GGCCGTC6301.4551915E-1111.02506525
CTGGTCG7201.8189894E-1210.61156419
CCGTCAG7351.8189894E-1210.39536427
GTCGGCC6705.0931703E-1110.36703422
CGTCAGG7605.4569682E-119.59705428
TAGAGCG5151.1443844E-69.44698857
CCGATCT4950.00551415337.016032717
GAGCGTT6451.914407E-47.007838259
GAGCGGG8400.00779511366.769535570
AGAGCGT7301.2016911E-46.666365658
GCGTTTG7055.637721E-46.414381561
Produced by FastQC (version 0.11.5)