FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6698248
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA234290.3497780315091349TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG28950.063.98630548
GATCGGA28950.062.9736251
TCGTATC29200.062.68542538
CTCGTAT29150.062.57850344
TCTCGTA29650.061.40051743
GCCGTCT30550.060.76253551
CGTATGC30700.060.55668346
ATGCCGT31000.059.98096549
TGCCGTC31000.059.8746750
ATCGGAA31100.059.8425562
TATCTCG30750.059.42293541
GTCACGA31600.058.8908429
ACACGTC31800.058.62569413
CGTATCT31200.058.55843439
ATTCGTA31750.058.51944436
CGTCTGA32150.058.2047416
TCGGAAG33050.057.3602033
TCGTATG32250.057.31952345
AATTCGT32650.057.221635
ATCTCGT31900.057.17696842
Produced by FastQC (version 0.11.5)