FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6698248
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN128080.19121418018562467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAT25350.019.86337334
GAGCGGG6650.018.2577489
GGGGGCG24050.017.49720656
GGGGCGG25550.016.75037857
AGCGGGG7950.015.2720910
CGGGGGA7550.015.22248161
GGGCGGG27550.015.15936458
GGGGGGC32150.015.14155355
TAGGGGG15250.014.8155250
GGGGCGA4356.511982E-1012.77225633
AGAGCGG5451.8189894E-1212.7299828
GGGGGGT51600.012.70878414
GCGGGGA9400.011.85321260
GGGCGAT3359.1373495E-611.40278234
CGGGTGA5501.7826096E-1011.39798961
AGGGGGG42000.011.09093351
GGCGGGG27800.011.01921459
GCGGGGG21750.010.84609111
CGGGGGG22700.010.5461912
CGGGTAA3004.6518238E-410.44815661
Produced by FastQC (version 0.11.5)