FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6322922
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3050.023.92848848
CGTATGC3600.020.27114346
CTCGTAT3600.020.26921844
TCTCGTA3800.019.20211443
GCCGTCT4450.017.96535351
TGCCGTC4150.017.58808350
TCGTATG4650.017.18775445
ATGCCGT4600.015.86650649
TATCTCG4600.015.86223841
ACCGCTC4650.015.68956632
CGCTCAT4900.014.88907934
GATCGGA4700.014.7865241
ACGGTAC2250.004180344814.76051370
ATACGCC2657.3342235E-414.62126370
CACCGCT5300.014.42075531
CCGCTCA5400.013.5104633
ACACGTC5750.012.68708113
ATCTCGT5850.012.47316742
ATCGGAA5651.8189894E-1212.2984462
CCGTCTT7150.011.6675652
Produced by FastQC (version 0.11.5)