FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8590997
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG40200.037.9562381
TATGCCG4550.032.84190848
AGCGATA5250.026.47350134
CTCGTAT5550.026.29692344
GATCGGA5650.025.2220331
CGTATGC5900.024.7370846
TCTCGTA6100.024.49512343
CGATAGA6100.023.92393736
GCCGTCT6250.023.91113951
CGTCTGA6600.022.11087416
TGCCGTC6900.021.65788350
ATGCCGT7150.021.3859349
GCGATAG6850.021.30440535
TCGGAAG7450.020.056413
CAGCGAT7650.019.5305833
ATCGGAA8300.019.259772
TCGTATG7650.019.0782745
ATCTCGT8150.018.75981342
ACACGTC7850.018.59046713
CGTCTTC8400.018.20572553
Produced by FastQC (version 0.11.5)