FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6504841
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATTCTGGTATATGATCTTGTTTATGAACTCTGGTTTTATTATTA315070.4843623387566276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTGT69200.060.48658470
GGCTTAC53400.048.89832358
GTTTGGC58950.044.81239354
GCTTACA59600.044.22199259
CTGGTAT60650.042.26880610
TATGATC61600.041.67332516
TCTGGTA62850.040.900089
TGGTATA63700.040.40854611
GAACTCT67350.039.40732231
TTAACTG73700.039.08442369
ACTCTGG68700.038.63382733
CTCTGGT68000.038.62282634
GGATGAT68250.038.3810232
TATGAAC70000.037.76455728
GATCTTG70500.037.15152719
TGGCTTA71850.036.92067757
ATCTTGT73150.035.90061620
GTTTATG73850.035.7487525
TGGATGA74450.035.2379231
CTTACAT75600.035.00351760
Produced by FastQC (version 0.11.5)