FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6504841
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGATTGAGGTTCGAGATATGAAAATATTACATGTCAAACAGTTAATTATGTAAGCCAAACTAATAATAATAAA165270.25407231322026164No Hit
TGGGATTGAGGTTCGAGATATGAAAATATTACCTGTCAAACAGTTAATTATGTAAGCCAAACTAATAATAATAAA121990.18753725110267877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTTCG36400.063.634269
GGTTCGA36500.063.3696510
CGAGATA38700.061.6551214
TCGAGAT39650.059.64634313
GTAAGCC47950.050.13695552
GAGGTTC48500.048.7571648
GTTCGAG48050.048.49746711
GTCAAAC52900.047.4127635
TGTAAGC53650.045.13153551
TTCGAGA52350.044.71224212
GGGATTG53900.044.362172
GGATTGA54650.043.8165253
GCCAAAC55750.043.4345256
GATTGAG56950.042.9053424
ATTGAGG62550.038.8490265
GATATGA65350.037.4691317
ACAGTTA66250.036.86804240
TAAGCCA68350.035.52512753
CAGTTAA70050.034.96779641
TGAGGTT68450.034.850557
Produced by FastQC (version 0.11.5)