FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6749526
Sequences flagged as poor quality0
Sequence length35-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN186170.275826776576607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGA7100.016.20413261
GGGGGAT24650.015.62996134
GGGGGCG24750.015.18735556
GGGGCGG27900.013.22918457
GGGCGGG28250.013.06889558
GGGGCGA4952.910383E-1112.61995233
GGGCGAT3501.151604E-611.90049634
GGGGGGC34250.011.78625755
GCGGGTA3258.121596E-510.72272160
TAGGGGG13000.010.70048550
GGGGATG26100.010.50688635
GATATCG3100.0030518769.96193769
CGGGGAA7402.910383E-119.89367861
GAGCGGG6251.8835635E-89.4296179
GGGGGGT59950.09.25449814
CGGGTGA6806.899427E-99.22855761
GGCGGGG28100.09.17473859
GGGATGT23900.08.5708236
AGGGGGG38600.08.56034351
GGGGGGA63300.08.55285933
Produced by FastQC (version 0.11.5)