FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5751156
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN165110.28709010849297084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAT16300.09.79518234
GGGGGCG14400.07.975966556
GGGCGGG17500.06.76664758
GGGGGGC22600.06.466907555
GGGGCGG16703.6379788E-126.46383557
GGGGGGA32600.06.38754933
GGCGGGG19004.0326995E-95.31730259
GGGGGAA28100.05.182525621
GCGGGGG14853.2696444E-65.162559560
GGGGGGT38400.05.057665314
Produced by FastQC (version 0.11.5)