FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10983854
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG175600.15987102523394794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT2104.659887E-818.19837237
TCGCGCA2901.8149658E-613.1774136
TCTCGCG3904.185251E-610.68897834
CGTATGC5007.638864E-79.73122446
GCGCATC4201.09553686E-49.09935138
CGGCCCG6356.126741E-48.77141770
TATGCCG4300.0013801358.08204448
TCTCGTA6158.551207E-46.781124643
CGCATCT7201.00964964E-46.755886639
TGCCCCG11255.408977E-46.43627170
CTCGCGC6100.0058577286.26455635
CCGGCCG10150.0086057166.0362970
GCCGTCT7050.0039739475.916069551
ACTCTCG7350.00635024775.67139332
TCGTATG7450.0073354765.59781645
CGTCTGA10503.9508007E-45.2927716
Produced by FastQC (version 0.11.5)