FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5646477
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGA4900.055.2391234
CGCGAAA5000.054.13769536
CCGCGAA5050.053.60025835
TATGCCG5550.048.2072248
CTCCGCG5800.047.2641633
TCTCGTA5950.044.93290343
CGTATGC6300.042.45394546
CTCGTAT6400.042.321744
GCGAAAT6600.041.54142837
TGCCGTC6550.040.8596650
GATCGGA6550.040.7959371
ACTCCGC6750.040.61108832
TCGTATG7200.037.6246345
ATCGGAA7600.036.5272452
ACACGTC7700.036.4960913
ATGCCGT7550.035.4440149
TCGGAAG8200.034.2758833
GCCGTCT7850.033.65651
ATCTCGT8000.032.98045342
CACTCCG8350.032.82728631
Produced by FastQC (version 0.11.5)