FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5646477
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN83500.14787981957599403No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAC800.002751893821.75922857
ACGTAAC2350.004417720314.63825270
CCGGTAA2500.001220609811.1217850
GGGCGGG14951.8189894E-126.98898358
GGGGGCG13101.0186341E-96.63971656
GCCATGG20350.05.9579892
GGGGCGG15355.4060365E-95.89696457
GGTTTGC17801.4733814E-105.84233510
CCATGGT22300.05.7486543
TTGCTGC23150.05.691538313
GGTGGTT21201.4551915E-115.5592077
GCTGCAC15854.1838757E-75.250378615
GAGCACT15300.0025766545.24617170
GGCGGGG16701.1062839E-65.00756259
Produced by FastQC (version 0.11.5)