FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6466182
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4050.030.03249548
TCTCGTA4850.025.07266843
CTCGTAT5200.024.72233244
TCGTATG5100.023.84619745
TTACTCG5100.023.83955635
GATCGGA5200.023.3834571
CGTATGC5600.022.33859846
TGCCGTC5500.022.11723550
GCCGTCT5850.020.79559151
ATGCCGT6200.020.17961149
TACTCGA6050.020.09615536
ATCGGAA6450.019.9260942
ATCTCGT6650.019.3308542
TCGGAAG6900.018.1232853
CGGAAGA7500.017.1361754
CGTCTGA7350.017.01119816
ACACGTC7550.016.10055413
CGTCTTC7900.015.841454553
CCGTCTT7950.015.7403652
AGGGGGG14950.015.1048411
Produced by FastQC (version 0.11.5)