FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6090876
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCA3200.055.34733636
CGCGCAT3450.049.3250837
TCTCGCG3950.044.83724634
GCGCATC3850.042.3969938
CTCGCGC4600.038.50249535
TATGCCG4450.038.25838548
TGCCGTC4850.037.97360650
CGTATGC4900.035.45044346
CTCGTAT5150.033.05278844
ACTCTCG5550.032.53663332
TCTCGTA5100.032.013243
ATGCCGT5650.031.36416649
GCCGTCT5800.029.95837451
TCGTATG6300.028.67476345
CGCATCT5800.028.14398639
GATCGGA6300.028.1159321
ACACGTC6600.027.88261813
CGTCTGA7050.026.59625616
CTCTCGC6850.025.85420233
CGTCTTC7400.024.42614753
Produced by FastQC (version 0.11.5)