FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18716978
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG11550.024.07052448
CGTATGC13100.021.75246
TGCCGTC13250.021.50868450
CTCGTAT12900.019.66397344
TTACTCG14900.019.35213935
GCCGTCT14750.019.32182551
TCTCGTA13900.017.24873743
TACTCGA16000.017.1536736
TCGTATG17050.016.91617845
ATGCCGT16700.016.85616949
GATCGGA17050.015.6924421
ACACGTC19300.014.75886813
ATCTCGT18050.014.43776942
ATCGGAA20750.014.2327192
CGTCTTC20850.013.83638853
CCGTCTT21700.013.29398452
CGTCTGA21450.013.11759716
TCGGAAG23600.012.5137033
CGGAAGA26300.011.2291164
ACTCGAT26100.010.64893937
Produced by FastQC (version 0.11.5)