FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6441346
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG8650.053.43115648
TCTCGTA8850.050.64477543
CTCGTAT9100.050.01899744
CGTATGC9850.046.918546
GATCGGA10150.045.8655361
TGCCGTC10200.045.65489250
AGCGATA10150.045.1736134
CGATAGA10300.044.51746736
GCGATAG10450.044.21018635
ATCTCGT10300.043.5138242
GCCGTCT10600.043.27837851
TCGTATG10850.042.59354845
ATGCCGT10900.042.08297749
CGTCTGA11400.041.43465416
ATCGGAA11900.039.99242
CAGCGAT11900.038.52923233
TCGGAAG12400.038.3768233
ACACGTC12500.037.23240313
ACAGCGA12550.037.08723432
CCGTCTT12600.036.68575752
Produced by FastQC (version 0.11.5)