FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6441346
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN91780.1424857475440692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG12050.08.65256550
GGGGGCG15150.08.25938956
GGGCGGG16000.08.04142158
CGGGGAA5452.1150749E-47.65958461
AGGGGGG22700.07.655527651
GGGGGGC20600.07.592587555
GGGGCGG16150.07.32009757
GCGGGGA6956.260943E-57.0065860
GGGGGGT24550.06.787658714
GGGGGAT16301.8189894E-126.60711934
TAGGCCC9900.0056048616.29095670
GCGGGGG13852.4359906E-86.014840611
CGGGGGG14809.833457E-85.62923512
ATAGGGG12302.2278058E-55.368744449
GGCGGGG17354.7540743E-75.01136459
Produced by FastQC (version 0.11.5)