FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8295282
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG97420.1174402509763984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG47450.044.6659051
TATGCCG6500.034.75092348
GATCGGA6200.034.7442171
CGTATGC7050.031.0517746
TCTCGTA6550.030.76605843
CTCGTAT6850.029.92693944
GCCGTCT7500.028.73072251
TGCCGTC7600.028.35131850
ATCGGAA7600.028.3396952
TTACTCG7750.028.23857735
TCGTATG8250.028.2195845
TACTCGA8100.027.87662536
TCGGAAG8200.026.2646333
ATGCCGT8700.025.56528749
ACACGTC9250.024.40852513
ATCTCGT9050.022.65051742
CGTCTTC10000.021.89810453
CGTCTGA10250.021.6874116
CGGAAGA10700.020.7772034
CCGTCTT10900.020.08854752
Produced by FastQC (version 0.11.5)