FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8253189
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG4800.039.088633
GAGCGGG11600.028.4370049
AGAGCGG10750.026.1607388
GAGAGCG11600.025.4393147
AGCGGGG14500.024.9060410
GGGGGCG38550.023.7873156
CGGGTAA4700.023.66177461
GCGGGTA4750.022.67829760
GGGGCGG41000.022.19805157
GGGGGGC50550.021.9876555
GGGGAGA59300.021.3633544
AGGGCGG12800.021.1740234
CGGGGGA9300.020.17929561
TAGTGCG3800.019.20141633
TAGGGGG24150.018.69242150
GGGAGAG63050.018.1665845
GTGCGGG7700.018.05055435
CGGGTGA8100.018.02019161
GGGCGGG43600.018.00816558
GGGGCCG13000.017.36870457
Produced by FastQC (version 0.11.5)