FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5685372
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA609.094947E-1152.1098243
AATCGCG1800.040.5241541
GATCGGA7050.039.4072651
ACACGTC7450.038.21329513
CCGGAGA7700.035.6271635
CACTCCG8050.035.36821431
ATCGGAA8000.035.1586532
ACTCCGG8500.033.49666632
TCGGAAG8650.033.31753
CGGAGAA8650.032.518136
CGGAAGA8950.032.2007144
ATCGCGT1850.031.92116542
CGTCTGA9150.031.11493116
TATGCCG3250.029.93949548
CACGTCT9750.029.55626314
CTCCGGA9850.029.25774633
TCCGGAG10150.029.07868834
CGCGTAT1155.2785254E-827.1886944
ACGTCTG10700.026.60762815
CGCGTTT1358.769348E-925.73415244
Produced by FastQC (version 0.11.5)