FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7750138
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG33500.033.4904481
TATGCCG4500.032.4364148
GATCGGA4850.027.9309731
TCTCGTA5650.027.670543
AGCGATA5500.027.15216634
TGCCGTC6050.025.85423750
CTCGTAT6050.025.84354844
CGTATGC5900.025.32507346
GCGATAG5800.025.14912235
CGATAGA6050.024.6855436
CGTCTGA6500.023.5048116
GCCGTCT6600.023.17530351
ACACGTC7050.021.1788513
ATCGGAA6650.020.8899352
ATGCCGT7200.020.7579949
TCGGAAG7050.020.6883223
TCGTATG7650.020.43875945
ACAGCGA8000.019.09913832
CAGCGAT7900.018.90243133
CCGTCTT8750.018.67496752
Produced by FastQC (version 0.11.5)