FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6830976
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG33700.038.7730331
GATCGGA4100.027.1230161
TATGCCG4300.024.24673548
CTCGTAT4550.023.67571344
CGTATGC4600.022.66376546
TCTCGTA4850.022.21042343
CGCTCAT5050.021.32814634
ACCGCTC5300.020.9765832
ATCGGAA5700.020.7267592
GTCCGCG850.003920799620.47045965
ATGCCGT5500.020.22119549
TGCCGTC5050.019.95885750
GTCACCG5650.019.06139629
GCCGTCT5300.019.01823851
TATCTCG6450.018.31653641
CGTCTGA6700.018.14824716
CACCGCT6150.018.07697931
TCGTATG6200.017.93566345
ACACGTC6950.016.99631113
ATCTCGT6500.016.57202742
Produced by FastQC (version 0.11.5)