FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7790213
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4750.032.1886348
CGTATGC5700.026.82213246
TCTCGTA5800.026.3557943
CTCGTAT5850.026.131744
GCCGTCT6350.025.72524851
TGCCGTC6400.025.52197650
ATCTCGT6600.023.68662542
TCGTATG6450.023.16308245
GATCGGA6350.022.4305111
ATGCCGT7100.021.5358949
CCGTCTT8450.019.3331152
CGTCTGA8300.019.24925816
ACACGTC8700.018.36376213
CGTCTTC9100.017.9522953
ATCGGAA8900.017.1725942
TCGGAAG9700.016.1137753
ACGTCTG11100.014.70649315
CGGAAGA10750.014.21676354
AATCTCG11900.013.72072841
CACGTCT12150.013.4355614
Produced by FastQC (version 0.11.5)