FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7667206
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7000.041.70432348
TCGTATC7250.039.7746838
TCTCGTA7600.037.94889543
CTCGTAT7700.037.00622644
CGTATGC7800.036.97922546
TGCCGTC7850.036.30478350
TATCTCG8200.035.1703141
GTCACGA8400.034.73637429
GCCGTCT8400.034.3430151
GATCGGA8350.034.119711
ATGCCGT9150.031.52574249
TCGTATG9250.031.1818745
ATCTCGT9600.030.04248442
ATCGGAA9600.030.0366022
ATTCGTA9600.030.03640636
ACACGTC9500.029.98199713
AGTCACG9850.029.9755528
GAATTCG10050.029.72795534
CGTCTGA9900.029.12241216
CGAATTC10200.028.6088533
Produced by FastQC (version 0.11.5)