FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5238586
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN78860.15053680516078194No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCG2350.00990552810.33105616
CTCTCGC3150.0084179558.80740921
GGGGGCG10851.1823431E-107.694466656
GGGGCGG12452.3501343E-96.709742557
GGGGGGC15952.1482265E-95.887306755
GGGCGGG11851.8614392E-65.8765458
GTTAAGG13500.00352597755.50372970
GGGGGGT19152.991328E-85.07121514
AGGGGGG17153.7547397E-75.06720651
Produced by FastQC (version 0.11.5)