FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6598668
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGA7900.046.17059742
CTGACCG8300.044.36235441
ACCGAGA10300.044.18453244
CCGAGAA10450.043.21884545
GACCGAG9300.039.2216743
CGAGAAG11800.038.27605446
TATGCCG5750.036.2578448
TGCCGTC5900.034.16360550
GATCGGA6050.033.877121
CTCGTAT6150.032.7634144
TCTCGTA6350.032.2773643
GCCGTCT6150.032.2117151
CGTATGC6600.031.58443646
ACCGCTC6500.031.52389332
TCGTATG7000.029.77825545
TATCTCG7050.029.07031241
CGCTCAT7150.028.6593934
GTCACCG7200.028.45540229
ATGCCGT7850.027.00256749
CGTCTGA7650.026.78094516
Produced by FastQC (version 0.11.5)