FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6598668
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTC9150.042.8077369
TCTCGGT10950.041.52723368
GACGTGA11350.040.96783
GTGACGT11100.040.6526761
TGACGTG11600.038.887132
CTTCTCG12050.036.4406366
TTCTCGG12150.036.32891567
CGTGAAA14050.032.113655
ACGTGAA14550.031.9603674
GGACTTA21150.022.1932659
GACTTAC20250.022.15312660
CTCGGTT5400.019.91165769
TTGGGAC24200.018.66942656
GGGACTT26450.017.34935258
GTGCAGC27450.016.5705219
CAATTGC29450.015.22001446
CTTGACT31500.014.55268632
AATTGCC31050.014.32404147
TCTTGAC30650.014.27535331
CTGGAAC32950.013.4942840
Produced by FastQC (version 0.11.5)