FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5299540
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC5000.045.1528738
TATGCCG5100.044.28423748
TAATGCG5400.042.44740734
TGCGCAT5450.042.06036837
AATGCGC5550.040.6751935
TCTCGTA5850.039.1944343
CTCGTAT5950.039.11994644
ATGCGCA5950.039.10923836
TGCCGTC5900.038.2839450
GATCGGA5750.038.060651
CGTATGC5950.037.95433846
ATCTCGT6500.035.80810542
GCCGTCT6350.035.57325451
ATGCCGT6350.035.0206849
CGCATCT6650.034.472839
ATCGGAA6900.033.7265432
CGTCTTC7200.031.86231853
ACACGTC7150.031.56947113
TCGTATG7200.031.36415345
TCGGAAG7450.030.3030823
Produced by FastQC (version 0.11.5)