FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6181768
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9100.057.28412648
CGGCTAT9600.055.00098834
CGTATGC10400.050.1167146
ACCGGCT10700.049.34627532
GCCGTCT10850.048.69126551
TCACCGG10950.048.536130
GTCACCG11000.048.3143129
ATGCCGT10800.048.26952749
TCTCGTA10400.047.77522743
TGCCGTC11000.047.7093950
CACCGGC11200.047.1433231
CTCGTAT10800.046.9724544
GATCGGA11050.046.8477671
ATCTCGT10750.045.89616842
ACACGTC12250.043.3825213
TCGTATG12000.043.1438745
CGTCTGA12700.042.66618716
ATCGGAA12550.042.0751882
TCGGAAG13300.040.2233053
CGTCTTC13250.039.87494353
Produced by FastQC (version 0.11.5)