FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5546540
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA5900.044.75588243
TATGCCG6150.042.37884548
CTCGTAT6300.041.91537544
GATCGGA6300.040.8090251
CGTATGC6550.040.31736846
ACCGCTC6800.038.3109932
GCCGTCT7150.036.45765751
TGCCGTC7250.036.43287350
CGCTCAT7100.036.20331234
TATCTCG7300.036.17027741
ATCTCGT7250.035.94149442
TCGTATG7550.035.4365945
CCGCTCA7550.034.96533633
CGTCTGA7850.034.07033516
ATCGGAA7750.034.0663832
GTCACCG7550.034.04396429
ATGCCGT7900.032.9923249
CACCGCT7950.032.76914631
ACACGTC8400.031.4251913
TCGGAAG9050.030.3236083
Produced by FastQC (version 0.11.5)