FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5546540
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN73250.1320643139687084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGCG2250.00706288210.813189544
TCCCACG5650.00178308689.022870
TATGCCC12502.2871606E-46.34403170
GGGGGCG12701.070659E-65.748467456
GGGCGGG14607.078597E-85.718136358
TGGGCGG7950.00239184275.687084757
CTGTCGC9304.4825335E-45.59882216
GGGGCGG13553.5046578E-65.39005957
Produced by FastQC (version 0.11.5)