FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4504811
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN52520.11658646722359717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA4700.059.1203236
CCGCGAA5050.055.7100435
TCCGCGA5200.054.10001434
CTCGTAT5500.051.17071544
TATGCCG5450.051.01795648
CGTATGC5950.047.30539346
TCTCGTA5950.047.29960643
CTCCGCG6100.046.1175333
GCCGTCT6200.045.9788451
GCGAAAT6250.045.01671637
ATGCCGT6300.044.68725249
GATCGGA6450.043.616481
TGCCGTC6400.043.44835750
TCGTATG6750.041.6965145
ACACGTC7250.040.71216613
ACTCCGC7100.040.60132632
ATCGGAA7250.040.2381172
ATCTCGT7350.038.76158542
CGTCTGA8100.036.8690216
TCGGAAG8300.035.56463
Produced by FastQC (version 0.11.5)