FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4504811
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN99870.2216963153393117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGCGG11350.08.581593557
GGGGGCG10607.094059E-117.87390156
GGGCGGG11101.5334081E-97.20954158
GGGGGGC14801.8189894E-127.04903355
AGGGGGG16400.06.99495651
ACTCCCC10500.0098194325.959357370
GGGGGGT20652.1464075E-105.377612614
Produced by FastQC (version 0.11.5)