FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5235926
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT1800.030.87147737
TCGCGCA1800.030.86911636
GCGCATC2500.022.22788838
TCTCGCG2600.021.3690934
TATGCCG2553.6379788E-1220.44450648
CTCGCGC3153.6379788E-1217.63898835
CTCGTAT3003.274181E-1117.3721844
TCTCGTA3400.017.37068643
CGCATCT3255.456968E-1217.09870139
GCCGTCT3201.5261321E-915.20894751
TGCCGTC3201.5279511E-915.20763850
ACTCTCG3907.2759576E-1215.13600432
CGTATGC3951.382432E-1014.07595346
ATCTCGT4202.5465852E-1114.06117742
GATCGGA3759.695214E-1013.8912341
CGTCTTC4356.366463E-1012.78956353
ACACGTC5054.1836756E-1112.37430713
CGTCTGA4651.87174E-911.94556816
ATGCCGT4401.0362783E-811.849249
CGAGTCT3350.004234254411.58189170
Produced by FastQC (version 0.11.5)