FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4645993
Sequences flagged as poor quality0
Sequence length35-76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN68880.14825678816132526No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACT450.00446050230.89333247
GACGAGG3400.00149933129.19627534
AATTACG3550.0022427648.7981036
GGGGGCG8201.7409548E-77.629722656
AGGGGGG14800.07.514512551
GGGGCGG9603.2407843E-76.88156757
GGGCGGG10704.2382453E-86.824908358
TAGGGGG8207.9071084E-56.357346550
GGCGGGG10501.0129628E-55.96212359
CGGGGGG9203.8709605E-45.659848712
GCGGGGG8600.0010107015.65028111
GGGGGGC13402.893712E-65.446619555
GGGGGGT19253.3087417E-85.04959814
Produced by FastQC (version 0.11.5)