FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9698512
Sequences flagged as poor quality0
Sequence length35-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA8200.039.8171841
AGGGGGG48150.039.4591331
ATCGGGG8650.037.74693742
AATCGGG9400.035.84100741
GAATCGG9750.033.84072540
CCGGAGA10500.033.0721135
ATCGGAA10150.032.846742
CGTCTGA11300.030.41965116
CACTCCG12350.028.67783731
ACACGTC12300.027.66450713
ACTCCGG12650.026.90047332
TCGGAAG13050.025.810923
CGGAGAA13850.025.07323836
CGGAAGA14100.024.873684
TCCGGAG14350.023.95686734
TCGGGGG10800.023.80115943
CTCCGGA14550.023.62707733
ACGTCTG15700.021.894415
CACGTCT17550.019.388614
CACACGT19600.017.89234512
Produced by FastQC (version 0.11.5)