FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7643168
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG115610.15125926840807372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9400.045.84739348
TCTCGTA10050.041.15032643
AGCGATA10600.040.6315634
GCGATAG10550.040.5002135
CGTATGC10600.040.00162546
CTCGTAT10300.039.81307644
CGATAGA10950.038.7050336
TGCCGTC11450.037.33977550
GCCGTCT11750.036.98048851
GATCGGA10750.036.194741
ATGCCGT11950.036.06571649
CAGCGAT12300.035.29658533
TCGTATG12100.034.7536945
ACACGTC12900.033.3788713
ATCGGAA12850.032.6993372
ATCTCGT12900.032.3260542
CCGTCTT14250.031.4707252
CGTCTGA13850.031.34006716
ACAGCGA14250.030.70905332
AGGGGGG26250.030.4392811
Produced by FastQC (version 0.11.5)