FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7643168
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN92940.12159879254256875No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG92540.12107544934247161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATGC4450.021.07076541
CGGATCT4500.020.81888429
GTTGGCG5050.019.24879636
AGCGGAT5550.017.50582527
GCGGATG5750.016.90956940
TTGGCGG6150.015.80695937
ACGCCTT6900.014.61029352
CCTCACG6800.014.30848548
TGGCGGA7150.013.11104738
AAGCGGA8600.012.5083726
GAAGCGG9300.011.193974525
CGCCTTG10450.09.980494553
GGAAGCG10100.09.62073724
GCGGATC15402.1827873E-116.53415128
GATGCCC17900.06.40282543
GGATGCC18601.8189894E-126.161494742
GGCGGAT16609.349606E-105.856807739
CTCAATT45100.05.69410670
GGCTGTA23801.2732926E-115.25135048
GCCTTGC21951.0695658E-95.06886954
Produced by FastQC (version 0.11.5)