FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8315228
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG36650.037.0785261
TATGCCG5650.031.98419648
TCTCGTA6300.029.78550743
CGTATGC6200.029.7065146
CTCGTAT6350.029.5515144
GATCGGA6450.029.0973721
TCGTATG6750.027.80047445
CGCTCAT7050.027.10664434
ACCGCTC7150.026.24141532
TGCCGTC7450.024.72457550
ATGCCGT7450.024.7241349
GCCGTCT7400.024.42315551
TATCTCG8400.023.99315841
TCGGAAG8350.023.3043843
GTCACCG8100.023.16288429
ATCGGAA8500.022.8945062
ATCTCGT9200.021.90692942
CCGCTCA8850.021.59329833
ACACGTC9100.020.99994913
CACCGCT10000.019.10960831
Produced by FastQC (version 0.11.5)