FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7956079
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7050.038.45311448
GCGCATC6700.033.7101638
CGTATGC8100.033.46718646
TCTCGTA6700.033.19529743
TAATGCG8300.033.0711634
TGCCGTC8550.032.52138550
GCCGTCT8600.032.3339351
TGCGCAT8250.031.16677537
AATGCGC8950.031.05756235
CTCGTAT7750.030.94066844
ATGCGCA9450.029.0468136
CGCATCT7950.028.84743939
ATGCCGT9400.028.84019949
TCGTATG9650.028.4505345
GATCGGA9300.028.0238551
ACACGTC10050.027.65614113
ATCGGAA10350.027.1927592
CGTCTGA10250.026.43859716
CGTCTTC10400.026.07087353
ATCTCGT8600.025.86096642
Produced by FastQC (version 0.11.5)