FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10807963
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG47850.040.074711
TATGCCG6600.028.39849748
CGTATGC7400.026.25808346
GATCGGA6100.026.149011
CTCGTAT7300.024.23502244
TCTCGTA7700.022.97318643
GTCACGA8450.022.97173329
ATCGGAA9450.022.0117322
TCGTATG9300.020.89062745
ATGCCGT9450.020.57303249
TGCCGTC9800.020.19777950
GCCGTCT9550.020.00511451
AGTCACG10450.019.23862828
CGTCTGA10700.018.7891316
TCGGAAG11450.018.1654593
ACACGTC11050.017.56702413
ATCTCGT10700.017.50239642
CATCTCG11350.016.49837741
ACGAGAT12500.015.80692332
ACGTCTG12800.015.43573515
Produced by FastQC (version 0.11.5)