FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12467656
Sequences flagged as poor quality0
Sequence length35-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG451930.3624819292415511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG150300.054.4534451
TATGCCG11150.046.12433648
GATCGGA11150.042.6829871
CGTATGC12300.041.80753746
CTCGTAT13250.038.80674444
GCCGTCT13750.037.41165551
TCTCGTA13900.037.2410943
TTACTCG13850.037.11590235
TGCCGTC14350.036.08650
ATCGGAA14550.034.6153562
ATGCCGT15250.034.40935549
TCGTATG15250.033.71882245
TACTCGA16100.032.3611536
ACACGTC16000.032.3429413
CGTCTGA16650.031.28814516
CGTCTTC16900.030.4439853
ATCTCGT17650.030.11517742
TCGGAAG17250.029.8001863
CCGTCTT18200.028.45778852
CGGAAGA18450.028.0499924
Produced by FastQC (version 0.11.5)