FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12467656
Sequences flagged as poor quality0
Sequence length35-76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG138560.11113556549843852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG69500.015.8346592
GAGGGGG84100.012.1326271
TGGGGGG128750.09.5992432
GTGGGGG138150.08.4409611
CACGGCC10250.0079341256.083917670
GATCGCT11200.00296102146.07404170
TCAGCCG14750.00187532435.380845570
Produced by FastQC (version 0.11.5)