FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6842590
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT25200.066.074899
CGCGAGT14800.060.8010529
CGGGATT31100.056.5015539
CGCGATT12500.053.2827879
CGCGTTA5200.053.1449627
GCGGATA18400.053.141089
GCGTAAT11600.051.3086289
ACGGATT13850.050.6470959
GGCGGAT30950.047.6194658
GGCGATT36800.047.5574159
GTCGCGA10650.046.574617
GGGGATT99850.046.195759
GCGCATT18400.045.8245549
CCGCATT11800.045.6352779
CGGTATT18250.045.4247069
TCGCGAG7000.043.526358
CGCGCAT9450.043.4888538
GGGTATT54550.042.9934549
GCGGGAT30000.042.986498
CGCGTGA9150.042.5934147
Produced by FastQC (version 0.11.5)