FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5493003
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3852797.013995805208919No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT21450.077.961199
GGGGATT86900.074.277329
CGCGATT8250.069.101979
GGGTATT40750.067.929079
CGGGATT24650.066.812589
GGCGATT21650.063.1971789
ACGGATT12000.056.4812559
GGCGAAT18150.054.443979
CGGTATT17650.054.191869
GACGATT10350.052.0212069
GCGGGAT19050.051.5393038
GGCGGAT22700.051.0654378
GCGCATT13400.050.5802279
CGCGAAT8150.050.519339
GCGTAAT10200.049.060149
GCGTATT16050.048.543599
GGGGGAT94700.048.494218
GCGGTAT12350.047.6999368
CGTTATT12100.047.1149759
ACGCGAT3100.046.996768
Produced by FastQC (version 0.11.5)