FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293588
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG24310.18792691336035894No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA20930.16179803770597748No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG15450.11943524522490934No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC14570.11263246103086917No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA13910.10753037288533908No Hit
GAACAATGGCATTAATACATACTTTCTGTGGCGAAAAGGAGTTTATCTTT13470.104128980788319No Hit
CAATTGTCATGTTGTTGTGCATGCCATTCAAAGGATTTTACAAACAATGA13050.10088219742298166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG450.0124.35061
TTACGCG501.8189894E-12111.915556
CGGCGAT502.6011548E-1097.92612
ACCCGTT1100.095.382567
CCGTTAG258.7751745E-488.48921110-114
CGCCGTA652.1827873E-1186.088887
CGTCCTA1202.910383E-1183.074165145
CGCACTA1100.082.664891
CGTCGAC1500.079.273518
CGCGATA652.0791049E-975.327777
TCGTGTT3400.072.004494
TCCCGTA601.0297481E-769.947229
CTTTACG3750.069.782295145
GCGCAAA1451.5825208E-1068.75104145
CGATCGT551.5808463E-467.96977145
ACGTGCG954.18108E-665.58487145
GTTCGCG555.0251147E-663.5883753
GCTAGCC3700.062.385361
AGCGTGA3300.061.468776
ACGCGCC956.2755134E-1058.902928
Produced by FastQC (version 0.11.5)