FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293588
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT8250.0129.63759
GCGGATT17700.0122.459839
GGCGATT19500.0121.3938759
TGCGATT8850.0120.042869
ACGGATT7050.0117.317349
GGGGATT46650.0115.395459
GACGATT6250.0115.221819
CGGGATT16750.0114.932349
GCGCATT11050.0112.274299
CGGTATT9350.0111.335749
ACGCGAT1550.0110.400978
CGGCATT13500.0109.327629
GTCGATT10050.0108.547229
TCGTATT6450.0108.332749
GCGTATT8650.0107.98139
GGGTATT22150.0107.836179
CGATATT5900.0107.5551769
CGTGATT8850.0107.152359
CCCGATT5200.0106.950949
TCGCATT7050.0106.192429
Produced by FastQC (version 0.11.5)